Centrifugal Partition Chromatography (CPC)

Centrifugal partition chromatography (CPC) is an underrated chromatographic technique that is based on the principles of Countercurrent Chromatography. In CPC separations are performed using a biphasic system, however instead of a single chamber as in a simple partitioning, the separations occur in thousands of chambers. The stationary phase is retained in these chambers through centrifugal forces, and the mobile phase is sprayed through under high pressure allowing for maximal interaction between the phases. The mobile phase elutes the components from the mixture at various rates relative to their partitioning coefficient in regards to the solvent system. Due to the large volume of the stationary phase in comparison to C18, sample loads of several grams can be processed.

CPC offers several modes that offer great selectivity. Conventional methods include isocratic and gradient based systems which make up a bulk of methods. Ion-exchange and ion-replacement allow for amazing selectivity based on pH.

Aside from great loading capacity and different selectivity, one of the crowning features of CPC is nearly total sample recovery. This is largely responsible to the lack of solid stationary phase which would otherwise have the capability to retain or catalyze the product. As a result, we have a system that offers great flexibility for highly labile substances.

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