Centrifugal partition chromatography (CPC) is an underrated chromatographic
technique that is based on the principles of Countercurrent Chromatography. In CPC separations are
performed using a biphasic system, however instead of a single chamber as in a simple partitioning,
the separations occur in thousands of chambers. The stationary phase is retained in these chambers
through centrifugal forces, and the mobile phase is sprayed through under high pressure allowing for
maximal interaction between the phases. The mobile phase elutes the components from the mixture at
various rates relative to their partitioning coefficient in regards to the solvent system. Due to
the large volume of the stationary phase in comparison to C18, sample loads of several grams can be
CPC offers several modes that offer great selectivity. Conventional methods include isocratic and
gradient based systems which make up a bulk of methods. Ion-exchange and ion-replacement allow for
amazing selectivity based on pH.
Aside from great loading capacity and different selectivity, one of the crowning features of CPC is
nearly total sample recovery. This is largely responsible to the lack of solid stationary phase
which would otherwise have the capability to retain or catalyze the product. As a result, we have a
system that offers great flexibility for highly labile substances.